The effects of 4β‐phorbol 12‐myristate 13‐acetate (PMA), bombesin and insulin on 6‐phosphofructo‐2‐kinase (PFK‐2) activity, on fructose 2,6‐bisphosphate concentration and on the phosphorylation state of PFK‐2 were investigated in primary cultures of hepatocytes from foetal and adult rats. By using the pre-optimized combination of enzymes contained in this kit, it is possible to minimize the lot-to-lot variation and improve the quality of the isolated hepatocytes. In addition, Worthington use-tests each lot by isolating hepatocytes from adult rat to assure performance, reliability, and consistent yield of viable cells.
Yamamoto, N., Imazato, K. & Masumoto, A. Growth stimulation of adult rat hepatocytes in a primary culture by soluble factor (s) secreted from nonparenchymal liver cell. Positive and negative regulation of JNK1 by protein kinase C and p42(MAP kinase) in adult rat hepatocytes. Jarvis WD(1), Auer KL, Spector M, Kunos G, Grant S, Hylemon P, Mikkelsen R, Dent P. Author information: (1)Department of Medicine, Medical College of Virginia, Richmond , USA.
Proliferation‐competent primary ‘monolayer’ cultures of adult rat hepatocytes that display a repertoire of differentiated functions have been characterized for growth state‐dependent expression of alcohol dehydrogenase activity (ADH; EC ). In an effort to reconstruct the cellular polarity normally found in the liver, adult rat hepatocytes were sandwiched between two layers of hydrated rat tail tendon collagen matrix.
Erin G. Schuetz, Steven A. Wrighton, Stephen H. Safe, Philip S. Guzelian, Regulation of cytochrome Pp by phenobarbital and phenobarbital-like inducers in adult rat hepatocytes in primary monolayer culture and in vivo, Biochemistry, . Normal rat hepatocytes were used as a positive control for C/EBP‐β. All analyses were performed at least three times. Results Albumin and α‐Fetoprotein Expression in DX‐Treated Acinar Cells. Exocrine acini from adult rat pancreas were purified by centrifugal elutriation and were cultured in bacteriological dishes to prevent cell adhesion.
Download full The Effect Of Butylated Hydroxytoluene Bht On The Metabolism Cytotoxicity And Genotoxicity Of Aflatoxin B In Primary Cultures Of Adult Rat Hepatocytes Book or read online anytime anywhere, Available in PDF, ePub and Kindle. Click Get Books and find your favorite books in the online library. The influence of four liver tumor promoters and two structurally related chemicals (non-promoters) on survival of normal adult rat hepatocytes was examined in .